• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:

    公开号:SE534158C2
    申请号:SE1050280
    申请日:2008-09-25
    公开日:2011-05-17
    发明作者:Jeffrey E Hartle；William C Carlson；Anthony R Shoaf
    申请人:Weyerhaeuser Company Law Dept；
    IPC主号:
    专利说明:

    534 158 2 inserted into a cotyledon barrier cavity with a larger diameter than the embryo itself, causes the cotyledons to bend away during the development of somatic embryos. This results in only the tips of the cotyledons coming into contact with the walls of the cotyledon barrier cavity, through which nutrients pass from the gametophyte to the embryo. The inventors' theory is that this caused the unacceptably low productivity of the seeds produced.
    Thus, there is a need for a method of preparing a material for use in a produced seed, which material improves the productivity of the produced seed.
    Summary This summary is intended to provide a selection of ideas in a simplified way, which ideas are described in more detail in the detailed description below.
    This summary is not intended to identify key features of the invention and is not intended to be used as an aid in determining the scope of the patent claims.
    A produced seed has been produced. The seed produced comprises a seed coat. which contains a nutrient, and a barrier, which is arranged inside the seed coat. The latch has a cavity. The seed produced also comprises an embryo which is arranged in the cavity and a filling material which is arranged in the cavity. The seed produced further comprises a closure. which is arranged on the seed coat to enclose the embryo in the seed coat.
    Description of the drawing The above aspects and many of the advantages obtained with the invention will appear from the following detailed description, referring to the accompanying drawing. In Fig. 1 is a planar side sectional view showing a seed produced having a packaging material in accordance with certain aspects of the present invention.
    Detailed Description A produced seed 20, which has a filler material 120 and is produced in accordance with certain aspects of the present invention, is best described with reference to Fig. 1. The produced seed 20 comprises a plug ("cylcap") 22, a seed coat 24, nutrient 26, such as a gametophyte, and a bottom end closure 28. The seed coat 24 is suitably formed of a length of tubular material. In one embodiment, the seed coat 24 is a tubular length of fibrous material, such as paper. The pipe lengths can be pretreated in a suitable coating material, such as wax.
    In other embodiments, the seed coat 24 is formed of a tubular length of biodegradable plastic material. One such material is a used polylactic acid ("PLA") and is sold by NAT-UR, Los Angeles, California. Another material within the scope of the invention is a polycaprolactone ("PCL") blend, such as Dow Tone P-787 (Dow Chemical Co., Midland, MI 48647) with a 1% Tegomer HS | 6440 plasticizer (Degussa Goldschmidt Chemical). Corp., 914 East Randolph Road, Hopewell, VA 23860).
    Such biodegradable plastic pipes are cut to suitable lengths for a produced seed. Furthermore, such biodegradable plastic pipes may or may not require a wax coating, as such pipes are already resistive to surrounding elements. It will be appreciated that, although cutting tubes is preferred, other embodiments, such as making tubes of suitable size for use as manufactured seeds, are also within the scope of the present invention.
    The plug 22, also known as a barrier, is suitably made of a porous material, such as a ceramic material or a porcelain material, which has sufficient hardness to prevent a germinating embryo from penetrating or cracking the material, and comprises an end closure portion 30 and a cotyledon barrier portion. 32. The cotyledon barrier portion 32 is suitably formed integrally with the end closure portion 30. The stopper 22 also has a longitudinally extending cavity 34 extending through the end closure portion 30 and partially through one end of the cotyledon barrier portion 32. The open end of the cavity 34 is known as a cotyledon barrier opening 36. The cavity 34 is sized to receive a plant embryo 42 therein.
    In some embodiments, since the plug 22 is suitably made of a porous material, it may be desirable to coat the plug 22 with a barrier material to reduce the rate of water loss and limit or reduce the microbial tree. Such barriers include wax. polyurethane, glaze, 10 15 20 25 30 534 158 4 nail polish and a coating, sold by H.B. Fuller, Minneapolis, MN under the product name PD124. The end closure portion 30 is suitably circular, seen from above, and has side walls 38. Although the end closure portion 30 is circular in the preferred embodiment, other embodiments and shapes, such as polygonal shape, square shape, triangular shape, oval shape and other shapes, are within the scope of present invention.
    In the embodiment shown in Fig. 1, the side walls 38 are defined by the thickness of the end closure portion 30 and have a diameter which is substantially equal to the inner diameter of the seed coat 24. In certain embodiments, the plug 22 is heat bonded to the seed shell 24. As a non-limiting example, the plug 22 may be heated to a predetermined temperature during manufacture that, when the seed shell 24 and the plug 22 join, heat transferred between the plug 22 and the seed shell 24 brings either the seed coat 24 or the plug 22 or both to melt, thereby connecting them to each other. In other embodiments, the plug 22 is first heat-welded with a primary end cap 44, after which the combined primary end cap plug is reheated in a separate step and the seed shell 24 is also heated so that the two units are interconnected. Other ways of connecting the plug 22 to the seed coat 24, for example by means of a wax connection or a hot melt adhesive, are also within the scope of the present invention.
    The side walls 38 may have a tapered portion 40. The tapered portion 40 may be a bevel at one end of the end closure portion 30. The tapered portion 40 facilitates the assembly of the plug 22 with the seed shell 24 during manufacture. Although a tapered portion 40 is preferred, other embodiments, such as a stopper without a tapered portion, are also within the scope of the present invention. An embryo 42 is located in the cavity 34 and is suitably enclosed therein by means of a top end closure 43.
    The top end closure 43 includes a primary end closure 44 and a secondary end closure 21. The primary end closure 44 is suitably made of a PCL material, described above, and has a centrally located opening 50. The opening 50 is dimensioned to correspond to the diameter of the plug cavity 34 and allow a germinating embryo 42 to pass therethrough. The primary end closure 44 can be securely attached to the end closure portion 30 by many different methods, including gluing or heat bonding.
    As a non-limiting example, the primary end closure 44 is so mated to a preheated plug 22 that the opening 50 is located above the cavity 34. The heat welds or connects the primary end closure 44 to the plug 22. It will be appreciated that the primary end closure The ring 44 can be attached to the plug 22 before or after its attachment to the seed shell 24. If the seed shell 24 is made of PCL, it is desirable, but not necessary, that the primary end closure 44 and the seed shell 24 have the same melting temperature.
    Another non-limiting example of attachment of the primary end closure 44 to the plug 22 includes an adhesive gasket. In this example, the primary end closure 44 is heat sealed or connected to the plug 22 with the opening 50 in a coaxial line with the cavity 34. In this process, a mold is used to bend the edges of the primary end closure 44 about the circumference of the end portion 30 of the plug 22. 44 and the seed coat 24 have different melting temperatures, a non-discoloring cyanoacrylate is used as an adhesive gasket to connect the primary end closure 44 and the seed shell 22.
    Heat is applied after gluing to dilute the glue seal by melting incongruences that typically occur during the production of the seed coat 24 and the formation of the adhesive joint. Thereafter, the stopper 22, including the primary end closure 44, is attached to the seed coat 24. As mentioned above, this method is also suitable for a stopper 22, which is already attached to the seed coat 24. The above method of attaching a primary end closure 44 to a seed coat 24 can finally be used for heat welding of compatible or incompatible materials.
    The secondary end closure 21 will now be described in more detail. The secondary end closure 21 is suitably made of a well-known sealing material, such as Parafilm®. The secondary end closure 21 is made and attached to the primary end closure 44 in a well known manner, such as by heat bonding or gluing. In certain embodiments, a sealing wax may be used to facilitate the connection between the PCL and the Parameter. The secondary end closure 21 also has a predetermined burst strength to allow a germinating embryo 42 to penetrate the top end closure 43.
    A tertiary closure 60 will now be described in more detail with reference to fi g 1. The tertiary closure 60 and the top end closure 43, as used in this embodiment, define an outer closure layer and an inner closure layer, respectively. Although the top end closure 43 has been described as including both a primary end closure 44 and a secondary end closure 21, it will be appreciated that the opening is not limited thereto. As a non-limiting example, the top end closure 43 may include only the secondary end closure 21, so such embodiments are also within the scope of the present invention.
    The combination of the tertiary closure 60 and the top end closure 43 provides a closure surface, the closure layer defined by the tertiary closure 60 being made of a predetermined material which degrades in structural integrity after a predetermined exposure to ambient conditions. The tertiary closure 60 also acts as an antimicrobial sealant for sealing and protecting around the embryo as it develops and protrudes from the interior of the seed coat 24, protecting the cotyledon barrier cavity. Suitable materials for making the tertiary closure 60 include water-soluble materials, waxes, environmentally degradable materials and biodegradable materials. Such materials, as well as materials which are equivalent in structure and properties, are thus within the scope of the present invention.
    As mentioned above, the seed coat 24 and the primary end closure 44 are suitably made of a polyester material, such as biodegradable plastic. One way of preparing the material for use in making components, such as the seed shell 24 and the primary end closure 44, in the produced seed 20 in accordance with the present invention comprises providing strips of material, such as plastic strips. Such plastics include, within the scope of the following claims, biodegradable plastics, such as PCL. 10 15 20 25 30 534 158 7 The material strips are blotted out by means of well-known blotting methods to improve the dimensional stability. In a non-limiting example, PCL strips mature at a temperature of about 63 ° C for a period of time of substantially between 35 minutes and 90 minutes. In one embodiment, the PCL strips expire for 45 minutes. The expiration time is sufficient to make the PCL strips dimensionally stable during heat welding. It should thus be understood that the temperature and time required for expiration are material-dependent and are within the skill of the art. It should also be understood that the strip of material does not need to be expired. Such embodiments are also within the scope of the following claims.
    Regardless of whether the strips of material expire or not, they are heat treated to remove toxins. In this process, and for PCL, the strips of material are placed in an oven and heat treated at a temperature of about 55 ° C for a period of eight days. It will be appreciated that a heat treatment of the material at other temperatures and for different time periods than at the specified specific temperature and during the specified specific time period is within the scope of the present invention. As a non-limiting example, the strips of material can be heat treated at a temperature which is about 10% lower than the melting temperature of the material. Furthermore, the friend treatment time can be as short as a few hours. Such time periods and temperatures are also within the scope of the following claims.
    The heat-treated strips of material are also subjected to a sterilization process. In this process, the strips of material are kept immersed in a sterilization mixture of bleach mixture for a sterilization pickling period. A bleach mixture is 10% bleach and 90% water. The sterilization pickling period is essentially between 10 minutes and 40 minutes.
    In a non-limiting embodiment, the strips of material are pickled in the bleach mixture for a sterilization period of 30 minutes. After the sterilization pickling period, the strips of material are subjected to rinsing in deionized water during a rinsing period. which can be up to and more than 72 hours. The strips of material are then air-dried in sterile air to evaporate. Finally, the material strips are processed to produce various components for the produced seed 20, such as the seed shell 24 and the primary end closure 44. The filler material 120 will now be described in more detail with reference to fi g 1.
    The filler material 120 is preferably an adsorbent, such as activated charcoal, Dowex resins, zeolites, alumina, clay, diatomaceous earth, silica gel and diatomaceous earth. When assembling the produced seed 20, the filler material 120 is placed in the cavity 34 of the plug 22 in any known manner, including manually. The filler material 120 is preferably, but not necessarily, placed in the cavity 34 so as to substantially center the embryo 42 in the cavity 34. A centering of the embryo 42 in the cavity 34 increases the surface area of the embryo 42 which is in functional contact with the nutrient 26. As it is used in this detailed description and the following claims refer to by "functional contact" a position where the embryo 42 absorbs nutrients from the food 26.
    The filler material 120 increases the surface area of the embryo 42 which is in functional contact with the food 26 by enclosing the embryo 42 and thereby providing a plurality of paths in which nutrients can pass from the food 26 to the embryo 42. Although the filler material 120 preferably substantially centers the embryo 42 in the cavity 34, the embryo 42 need not be placed in this way. The filler material 120 only needs to position the embryo 42 in the cavity 34 in such a way that it is in functional contact with the food 26. It is also not necessary for the filler material 120 to "enclose" the embryo 42. The filler material 120 can thus completely or partially enclose the embryo 42 In other embodiments within the scope of the following claims, the filling material 120 only needs to fill, either completely or partially, one or two sides of the space between the embryo 42 and the walls of the cavity 34.
    One way of preparing the filler material 120 for insertion into the cavity 34 involves combining approximately 7.0 g of activated charcoal with 246 ml of a nutrient, such as KE64 agent, to provide a mixture. Such a composition of the KE64 agent is given in Table 1. 10 534 158 9 Medium §_ | L & Additive Component concentration amount for LngL) 300 ml NH4NO3 301.1 2.2 ml H3BO3 10.0 0.75 ml (NH4) 2MoO4 0.06 CaCl2-2H2O 299.2 1.00 ml KH2PO4 1800.0 7.5 ml MgSO4-7H2O 1000.0 MnCl2.4H2O 6.0 0.75 ml ZnSO4-7H2O 0.8 CuCl2-2H2O 0.5 Ferric citrate 60 nlg / l 0.75 ml TABLE 1 Then 30 ml of a 50% sucrose solution and organic masses are added to the mixture. The composition of such an organic mass is given in Table 2. 534 158 10 Medium y _S | ¿t- Quantity of addition Component concentration concentrated for 300 ml mM (mg / l) complete media Myoinositol 0.5549 100.0 3.0 ml Thiamine-HCl 0.0030 1.0 Pyridoxine-HCl 0.0012 0.25 Nicotinic acid 0.0081 1.0 Riboflavin 0.0021 0.125 Ca-pantothenate 0.50 Biotin 0.0003 0.0010 Folic acid 0.807 0.1250 L-asparagine 1.8255 106.7 3.0 ml L-glutamine 0.3646 266.7 L-lysine-2HC | 0.7612 53.3 DL-serine 0.4631 80 L-proline 1.5310 53.3 L-arginine-HCl 0.4552 266.7 Urea 13.3200 800 L-valine 0.5983 53.3 3.0 ml L-alanine 0.2203 53.3 L-Ileucine 02448 80 L-threonine 0.3226 26.7 L-phenylalanine 0.1720 53.3 L-histidine 0.1308 26.7 L-tryptophan 0.2035 26, 7 L-isoleucine 1.2930 26.7 L-methionine 0.7100 26.7 LIL-glycine 0.0003 53.3 L-tyrosine 0.2242 53.3 0.75 ml L-cysteine 0.6098 26.7 0.75 ml TABLE 2 10 534 158 11 Then the mixture is autoclaved for about 25 minutes. The mixture is filtered through well-known filter paper, and the filler material 120 (eg charcoal) is taken from the filter paper. This filler material 120 is then dried until it becomes surface material. Illustrative embodiments have been illustrated and described, but it should be understood that various changes may be made within the scope of the invention.
    权利要求:
    Claims (10)
    [1]
    A seed produced, characterized in that it comprises: a) a seed coat (24), which contains a nutrient (26); b) a latch (22) disposed within the seed shell (24) and having a cavity (34); c) an embryo (42) disposed in the cavity (34); d) a filler material (120) disposed in the cavity (34); and e) a closure (43) disposed on the seed coat (24) for enclosing the embryo (42) in the seed coat (24).
    [2]
    A seed produced according to claim 1, wherein the filler material (120) is arranged in the cavity (34) to place the embryo (42) in functional contact with the nutrient (26).
    [3]
    A seed produced according to claim 2, wherein the filler material (120) is an adsorbent.
    [4]
    A seed produced according to claim 2, wherein the filler material (120) is charcoal.
    [5]
    A seed produced according to claim 1, wherein the filler material (120) is pretreated charcoal.
    [6]
    The seed produced according to claim 1, wherein the filler material (120) is an adsorbent.
    [7]
    A seed produced according to claim 1, wherein the filler material (120) is charcoal.
    [8]
    The seed produced according to claim 1, wherein the filler material (120) is pretreated charcoal.
    [9]
    The seed produced according to claim 1, wherein the filler material (120) substantially centers the embryo (42) in the cavity (34). 534 158 13
    [10]
    The seed of claim 1, wherein the filling material (120) substantially centers the embryo (42) in the cavity to increase the surface area of the embryo (42) which is in functional contact with the nutrient (26).
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    法律状态:
    2018-05-02| NUG| Patent has lapsed|
    优先权:
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    US97717207P| true| 2007-10-03|2007-10-03|
    PCT/US2008/077696|WO2009045851A1|2007-10-03|2008-09-25|Manufactured seed having packing material|
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